Reporter Gene Assays: Methods and Protocols by Robert Damoiseaux (English) Hardc

Description: Reporter Gene Assays by Robert Damoiseaux, Samuel Hasson This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation. FORMAT Hardcover LANGUAGE English CONDITION Brand New Publisher Description This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation. The chapters in this book cover practical topics such as how to integrate reporter constructs into cellular models, viral delivery, splicing applications, in vivo imaging, and a guide to the use of multi-cistronic constructs. Additionally, chapters also include detailed mechanistic uses of reporter genes in cellular pathways, and a look at project and data management of screening applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Reporter Gene Assays: Methods and Protocols is a valuable resource for anyone who is interestedin learning more about reporter genes. Back Cover This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation. The chapters in this book cover practical topics such as how to integrate reporter constructs into cellular models, viral delivery, splicing applications, in vivo imaging, and a guide to the use of multi-cistronic constructs. Additionally, chapters also include detailed mechanistic uses of reporter genes in cellular pathways, and a look at project and data management of screening applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Reporter Gene Assays: Methods and Protocols is a valuable resource for anyone who is interested in learning more about reporter genes. Table of Contents Genome-Edited Cell Lines for High-Throughput Screening.- High-Throughput Firefly Luciferase Reporter Assays.- Using the 2A Protein Co-Expression System: Multi-Cistronic 2A Vectors Expressing Gene(s) of Interest and Reporter Proteins.- Developing Mammalian Cellular Clock Models Using Firefly Luciferase Reporter.- High-Throughput Screening Method to Identify Alternative Splicing Regulators.- High-Throughput Screening of a Luciferase Reporter of Gene Silencing on the Inactive X Chromosome.- Making It All Work: Functional Genomics and Reporter Gene Assays.- Reporter Gene Assays Using Transfectable Functional Genomics Libraries.- Reporter Gene Assays Using Viral Functional Genomics Libraries.- Using YFP as a Reporter of Gene Expression in the Green Alga Chlamysomonas reinhardtii.- Gene Expression Analysis by Arylsulfatase Assays in the Green Algo Chlamydomonas reinhardtii.- Endogenous Locus Reporter Assays.- High Content Reporter Assays.- Data Mining and Computational Modeling of High-Throughput Screening Datasets.- Intravital Imaging by Two-Photon Excitation Microscopy of Human Melanoma Cells in the Mouse Ear Skin. Feature Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Details ISBN1493977229 Publisher Humana Press Inc. Series Methods in Molecular Biology Year 2018 Edition 1st ISBN-10 1493977229 ISBN-13 9781493977222 Format Hardcover Imprint Humana Press Inc. Subtitle Methods and Protocols Place of Publication Totowa, NJ Country of Publication United States Edited by Samuel Hasson Language English DEWEY 572.8 Pages 234 Illustrations 42 Illustrations, color; 11 Illustrations, black and white; XII, 234 p. 53 illus., 42 illus. in color. Publication Date 2018-04-19 Short Title Reporter Gene Assays DOI 10.1007/978-1-4939-7724-6 Series Number 1755 AU Release Date 2018-04-19 NZ Release Date 2018-04-19 US Release Date 2018-04-19 UK Release Date 2018-04-19 Author Samuel Hasson Edition Description 1st ed. 2018 Alternative 9781493992683 Audience Tertiary & Higher Education We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:130978541;

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