M9 Minimal Salts, 5X For Cultivating of Escherichia coli in Molecular Biology

Description: M9 Minimal Salt, 5X M9 Minimal Salts is a highly-referenced microbial growth medium used for the cultivation of E. coli. This buffered minimal microbial medium contains only salts and nitrogen, so it is traditionally supplemented with glucose, amino acids and vitamins as needed. ApplicationM9 Minimal Salts 5x are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for maintaining positive selection pressure on E. coli that produce essential amino acids and for maintenance of F′-containing bacteria strains for M13 propagation.in GMML minimal media to propagate phage-resistant DH10B cellsin a selective agar medium for the isolation of sulphate reducing bacteriain M9 minimal medium which is used for cell motility assayPreparation: 1. Dissolve 56.4 g in 1L of distilled water. 2. Autoclave for 15 minutes at 121°C. This convenient 5X concentrate can be stored and diluted as needed to prepare 5 L of 1× M9 minimal salts. For M9 minimal medium: 1. Aseptically dilute 200 ml of M9 minimal salts, 5X concentrate with 800 ml of sterile water. If necessary, cool to 45-50°C. 2. Aseptically add 20 ml of sterile 1 M glucose and 2 ml of sterile 1 M magnesium sulfate to prepare 1 L of M9 minimal medium. 3. If desired, aseptically add 0.1 ml of 1 M sterile calcium chloride to the M9 minimal medium. The M9 minimal medium may also be supplemented with the appropriate amino acids.

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Location: Thomastown, VIC

End Time: 2025-01-05T04:16:06.000Z

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